Composite

Part:BBa_K3166056:Design

Designed by: Yijia Ren   Group: iGEM19_SEFLS_Shanghai   (2019-10-16)


pBBR1MCS-2-MK-PMK-PMD-Idi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3142
    Illegal SpeI site found at 4279
    Illegal PstI site found at 847
    Illegal PstI site found at 1138
    Illegal PstI site found at 1483
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4279
    Illegal PstI site found at 847
    Illegal PstI site found at 1138
    Illegal PstI site found at 1483
    Illegal NotI site found at 1975
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3142
    Illegal SpeI site found at 4279
    Illegal PstI site found at 847
    Illegal PstI site found at 1138
    Illegal PstI site found at 1483
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3142
    Illegal SpeI site found at 4279
    Illegal PstI site found at 847
    Illegal PstI site found at 1138
    Illegal PstI site found at 1483
    Illegal NgoMIV site found at 81
    Illegal NgoMIV site found at 2013
    Illegal AgeI site found at 2466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 972
    Illegal BsaI site found at 3090
    Illegal BsaI.rc site found at 910
    Illegal SapI.rc site found at 414
    Illegal SapI.rc site found at 1515


Design Notes

PCR


Source

pBBR1MCS-2-MK-PMK-PMD-Idi

References